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Diversity and distribution of the Euastrum humerosum / didelta (Desmidiales) species complex
Kupčíková, Eva ; Šťastný, Jan (advisor) ; Kollár, Jan (referee)
From 23 European and North American strains Euastrum humerosum/didelta species complex (Desmidiales) I have obtained 3 lineages in molecular marker trnGucc group II intron. I did not acquire significant and homogenous results in SSU intron. It is probable that the lineages of trnGucc intron are very young. SEM revealed one central pore in cell wall and five bulges. The lineages were significantly different in their shape and dimensions. The lineages differentiated mainly in their length and breadth of the cells. The lineages inside morphotype E. humerosum differentiated in shape and dimensions of the polar lobe. The lineages inside morphotype E.didelta differentiated in shape and dimensions of the neck. That was ascertained using the geometric morphometrics with the measuring. Linear discriminant analysis of the desmids from literature revealed that is possible to partly discriminate groups of the varieties (E. humerosum var. parallelum and E. didelta in lineage A; two formae E. didelta f. val Piora and E. didelta f. latior in lineage B; E. didelta var. inermiforme and E. humerosum var. affine in lineage C). Key words: desmids, species, hidden diversity, molecular phylogenetics, geometric morphometrics, Euastrum, scanning electron microscopy
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Diversity and distribution of the Euastrum humerosum / didelta (Desmidiales) species complex
Kupčíková, Eva ; Šťastný, Jan (advisor) ; Kollár, Jan (referee)
From 23 European and North American strains Euastrum humerosum/didelta species complex (Desmidiales) we have obtained 3 lineages in molecular marker trnGuuc group II intron. We did not acquire significant and homogenous results in SSU intron. It is probable that the lineages are very young. The lineages were significantly different in their shape and dimensions. We used the geometric morphometrics with the measuring of the lengths and breath of the semicells, isthmus and their ratios. The lineages differentiated in their dimensions and shape variable polar lobes and neck. SEM revealed the one central pore in cell wall on the contrary, with variant E. didelta var. bengalicum, which has two pores according the literature records. Key words: desmids, species, hidden diversity, molecular phylogenetics, geometric morphometrics, Euastrum, scanning electron microscopy
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Cryptic diversity of freshwater alga Synura sphagnicola (Chrysophyceae, Stramenopiles)
Slámová, Pavla ; Škaloud, Pavel (advisor) ; Dvořák, Petr (referee)
A common alga of oligotrophic slightly acidic fresh water habitats, Synura sphagnicola, has never been suspected for a presence of cryptic diversity. The publicly available SSU rDNA sequences showed very slight genetic differences between the S. sphagnicola isolates. However, I suspected that if any cryptic lineages exist, the sequencing of ITS region will show the differences between them. A total of 37 S. sphagnicola sequences (36 from Europe and 1 from Korea) were analyzed in this thesis. The ITS rDNA sequencing clearly recognized the presence of two distinct cryptic species, referred here as lineage SP1 and SP2. The morphological analysis of 14 cultivated strains (6 belonging to the lineage SP1 and 8 to the lineage SP2) validated the genetic distinction. The statistical analyses showed that 4 morphological aspects (length of a scale, width of a scale, length of a spine, length of a rim) were found to have significant differences in length of measured factors between species. The most visible difference is in the length of a spine that is connected to the scale. The analysis of published S. sphagnicola scales showed that the two lineages can be morphologically distinguished also in natural conditions. According to the morphological analyses, the lineage SP2 (species with a longer spine)...
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